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Erence among moderate-dosage insulin, low-dosage insulin and advanced high-dosage insulin(p> 0. can you buy viagra australia 05). (3)compared with dm groups, all insulin groups decreased fbg and increased serum level of insulin, and there were significant differences(p<0. viagra young men 05). The serum level of insulin was significantly inversely correlated with ai of colonic smc(r=-0. order viagra online 709, p<. 01). All igf-1 groups increased serum level of igf-1, and there were significant differences(p< 0. 01). generic viagra overnight shipping The serum level of igf-1 was significantly inversely correlated with ai of colonic smc(r=-0. 857, p<0. viagra without a doctor prescription 01). should i take 50mg or 100mg viagra All igf-1 groups did not decrease fbg. should i take 50mg or 100mg viagra Conclusion:both insulin and igf-1 can improve the apoptosis of colonic smc in diabetic colon dysmotility, and the effects of early high-dosage insulin were better than those of moderate-dosage insulin, low-dosage insulin and advanced high-dosage insulin. Partiv the effects of high glucose, insulin and igf-1 on pi3k/akt pathway of colonic smcobjective:to explore the effects of high glucose, insulin and igf-1 on proliferation, apoptosis and pi3k/akt pathway of colonic smc. generic viagra Methods:in vitro culture of the rat colonic smc is performed by the means of tissue sticking. The colonic smc were devided into ten groups including normal control group, high glucose group(33. 3 mmol/l glucose), high glucose+ly10 group(33. 3 mmol/l giucose+10î¼mol/l ly294002), mannitol group(33. 3 mmol/l mannitol), ins group(100 nmol/ l insulin), ins+ly10 group(100 nmol/l insulin+10î¼mol/l ly294002), ins+ly50 group(100 nmol/l insulin+50î¼mol/l ly294002), igf-1 group(100î¼g/l igf-1), igf-1+ly10 group(100î¼g/ligf-1+10î¼mol/l ly294002), igf-1+ly50 group(100î¼g/l igf-1+50 umol/l ly294002). We used mtt to analyze the proliferation of smc and flow cytometry to analyze the apoptosis. Western blot was performed to analyze the protein expression levels of akt, p-aktthr308, p-aktser473 and caspase-9 of colonic smc. generic viagra canada Results:(1)sufficient rat colonic smc were obtained by culture in vitro. (2)compared with normal control group, the proliferation was decreased and apoptosis was enhanced in high glucose group and high glucose+ly10 group, and there were significant differences(p<0. buy viagra canada 01). The changes above became more significant in hig.
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